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Fisher Scientific viability stain ghost dye red 780
Viability Stain Ghost Dye Red 780, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viability+stain+ghost+dye+red+780/viability+stain+ghost+dye+red+780/pmc11398865-154-3-9
Average 90 stars, based on 1 article reviews
viability stain ghost dye red 780 - by Bioz Stars, 2026-09
90/100 stars

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Staining:

Article Title: Lymphoid origin of intrinsically activated plasmacytoid dendritic cells in mice
Article Snippet: Single- cell suspensions were stained with the following antigen- specific monoclonal antibodies (clones in parenthesis): anti CD45R/B220- BV605 (RA3- 6B2), CD19Alexa Fluor 700 (6D5), CD86- APC- Cy7 (GL- 1), I- A/I- E- Pacific Blue (M5/114.15.2), IL7RBV421 (A7R34), cKit- PE- Cy7 (ACK2), CD11b- PerCP- Cy5.5 (M1/70), CD3e- PerCP- Cy5.5 (500A2), Gr1PerCP- Cy5.5 (RB6- 8C5), CD4- PerCP- Cy5.5 (RM4- 5), CD8- PerCpCy5.5 (53–6.7), NK1.1- PerCp- Cy5.5 (PK136), Sca1- BV711 (D7), CD135- APC (A2F10), CD150- BV605 (TC15- 12F12.2), Cd79a- FITC (24C2.5) (Invitrogen), Anti- CD11c- PerCP- Cy5.5 (N418) (eBioscience), Anti- AXL- APC (175128) (R&D Systems), Anti- CD23 BV510 (B3B4) (BioLegend), Anti- CD267- BV421 (8F10) (BD Biosciences), Anti- CD138- APC (281- 2) (BioLegend), Anti- CD83- PeCy7 (Michel- 19), and Anti- PDCA1- PE or Biotin (JF05- 1C2.4.1) (Miltenyi Biotech), in D- PBS/2% (vol/vol) FBS FACS buffer. .. In some experiments, viability stain Ghost Dye Red 780 (Fisher Scientific) was used to stain cells in PBS prior to antibody staining according to the manufacturer’s protocol. .. Flow cytometry data was analyzed with FlowJo, while imaging flow cytometry was performed on ImageStream X and analyzed on IDEAS 6.0 (Amnis), by pre- filtering out of focus cells and performing automatic software guided compensation with single color controls.

Article Title: Lymphoid origin of intrinsically activated plasmacytoid dendritic cells in mice
Article Snippet: Single-cell suspensions were stained with the following antigen-specific monoclonal antibodies (clones in parenthesis): anti CD45R/B220-BV605 (RA3-6B2), CD19Alexa Fluor 700 (6D5), CD86-APC-Cy7 (GL-1), I-A/I-E-Pacific Blue (M5/114.15.2), IL7R-BV421 (A7R34), cKit-PE-Cy7 (ACK2), CD11b-PerCP-Cy5.5 (M1/70), CD3e-PerCP-Cy5.5 (500A2), Gr1-PerCP-Cy5.5 (RB6-8C5), CD4-PerCP-Cy5.5 (RM4-5), CD8-PerCpCy5.5 (53–6.7), NK1.1-PerCp-Cy5.5 (PK136), Sca1-BV711 (D7), CD135-APC (A2F10), CD150-BV605 (TC15-12F12.2), Cd79a -FITC (24C2.5) (Invitrogen), Anti-CD11c-PerCP-Cy5.5 (N418) (eBioscience), Anti-AXL-APC (175128) (R&D Systems), Anti-CD23 BV510 (B3B4) (BioLegend), Anti-CD267-BV421 (8F10) (BD Biosciences), Anti-CD138-APC (281-2) (BioLegend), Anti-CD83-PeCy7 (Michel-19), and Anti-PDCA1-PE or Biotin (JF05-1C2.4.1) (Miltenyi Biotech), in D-PBS/2% (vol/vol) FBS FACS buffer. .. In some experiments, viability stain Ghost Dye Red 780 (Fisher Scientific) was used to stain cells in PBS prior to antibody staining according to the manufacturer’s protocol. .. Flow cytometry data was analyzed with FlowJo, while imaging flow cytometry was performed on ImageStream X and analyzed on IDEAS 6.0 (Amnis), by pre-filtering out of focus cells and performing automatic software guided compensation with single color controls.



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